10.11582/2023.00103
Title Downward vertical flux of size-fractionated Chlorophyll-a and phaeopigments in the northern Barents Sea during December 2019, Nansen Legacy cruise 2019711 Q4
Subject
Domain: Natural sciences, Field: Biology, Subfield: Marine Biology
Domain: Natural sciences, Field: Biology, Subfield: Biochemistry
Domain: Natural sciences, Field: Chemistry, Subfield: Marine chemistry
Published 2023-09-01
Creator
Yasemin V. Bodur
Martí Amargant-Arumí
Marit Reigstad
Dataset (21.25 KB)
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Description
Samples for sinking mass flux of size-fractionated (>0.7µm and >10µm) Chlorophyll-a and Phaeopigments were collected with short-term drifting sediment traps between 75° and 85°N in the northern Barents Sea with R/V Kronprins Haakon in December 2019 as part of the Nansen Legacy project (cruise 2019711, Q4). High-density bottom water was collected at the station and filtered (Whatman GF/F 0.7µm), and then filled into the trap cylinders (KC Denmark, aspect ratio >6) prior to their deployment. Afterwards, the sediment traps were deployed with 2 or 4 cylinders at each depth (30, 40, 60, 90, 120, 200m) for a duration between 28 to 42 hours. Depending on the sea ice conditions, the sediment traps were deployed in open water, anchored to a sea ice floe or within an ice lead. At one station, a sediment trap was deployed though a sea ice hole for 12 hours. Upon retrieval, the content of the cylinders from each depth was pooled and stored at 4°C in dark until further analyses. Between 100-300ml were filtered onto 0.7µm GF/F filters (Whatman) for the analysis of Chla and Phaeopigments, depending on the amount of material in the traps. To quantify the contribution of larger cells, additional subsamples for Chla were filtered onto 10µm polycarbonate filters (Millipore). After filtration, both filters were immediately transferred to a tube with 100% methanol and stored between 12-24h at 4°C for pigment extraction. Size-fractionated Chla (0.7 and 10µm) was measured with a pre-calibrated Turner Design AU-10 fluorometer before and after acidification with 5% HCl. Chla and phaeopigments were calculated according to Holm-Hansen & Riemann, 1978. Holm-Hansen, Osmund, and Bo Riemann. “Chlorophyll a Determination: Improvements in Methodology.” Oikos 30 (1978). https://doi.org/10.2307/3543338.
Science publication
Paper in preparation (primary)
Rights Holder
Marit Reigstad
Data Manager
Luke Marsden
Depositor
Yasemin V. Bodur
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