Chr. Michelsen Institute | Natural sciences | University of Bergen | University of Oslo

Raw data for Frei, D. M. et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer

Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT.

Dominik Michael Frei, University of Bergen, Tanja KögelDOI : 10.11582/2016.00001License: Creative Commons Attribution 4.0

959.3 GBBiota

Cell Biology

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Field	Value
Theme	Biota
Subjects	Natural sciences
Type	dataset
Language	English
Status	COMPLETE
Projects
Dataset Size (MB)	959303.861602
State	active
Last Modified	2025-11-11T16:49:08

Identifiers

Field	Value
Identifier	903b14c8-5b2b-4a60-8b44-dbb1605ee7c2
Other Identifier/DOI	10.11582/2016.00001

Personnel (first name, last name, organisation, email)

Field	Value
Contact points	Contact point 1 Type person First name Dominik Michael Last name Frei Organisation University of Oslo Email d.m.frei@ibv.uio.no ORCID Name Acronym Contact email Homepage URL ROR Contact point 2 Type person First name Tanja Last name Kögel Organisation NIFES (The National Institute of Nutrition and Seafood Research) Email tko@nifes.no ORCID Name Acronym Contact email Homepage URL ROR
Creators	Creator 1 Type person First name Dominik Michael Last name Frei Organisation University of Oslo Email d.m.frei@ibv.uio.no ORCID Name Acronym Contact email Homepage URL ROR Creator 2 Type organisation First name Last name Organisation Email ORCID Name University of Bergen Acronym Contact email post@biomed.uib.no Homepage URL ROR Creator 3 Type person First name Tanja Last name Kögel Organisation NIFES (The National Institute of Nutrition and Seafood Research) Email tko@nifes.no ORCID Name Acronym Contact email Homepage URL ROR Creator 4 Type person First name Erlend Last name Hodneland Organisation Christian Michelsen Research (CMR) Email erlend.hodneland@cmr.no ORCID Name Acronym Contact email Homepage URL ROR
Contributors	Contributor 1 First name Tanja Last name Kögel Organisation NIFES (The National Institute of Nutrition and Seafood Research) Email tko@nifes.no ORCID Type Related Person Contributor 2 First name Erlend Last name Hodneland Organisation Christian Michelsen Research (CMR) Email erlend.hodneland@cmr.no ORCID Type Related Person Contributor 3 First name Dominik Michael Last name Frei Organisation University of Oslo Email d.m.frei@ibv.uio.no ORCID Type Related Person Contributor 4 First name Ivan Last name Rios Mondragon Organisation University of Bergen Email Ivan.Rios-Mondragon@uib.no ORCID Type Related Person Contributor 5 First name Anne Pieretta Last name Burtey Organisation University of Oslo Email a.p.burtey@ibv.uio.no ORCID Type Related Person
Dataset owner	Owner 1 Organisation University of Oslo ROR 01xtthb56 Owner 2 Organisation University of Bergen ROR 03zga2b32 Owner 3 Organisation Chr. Michelsen Institute ROR 02w7rbf39
Publisher	NIRD RDA

Constraints

Field	Value
License	CC-BY-4.0
Access Rights	public

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Spatial
Temporal

Reference Dates

Field	Value
Release date	2016-04-22

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Release date

2016-04-22

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